for Technology Evaluations, Site Closure Strategies & Bench Testing


EBS ServicesSince 1989, Environmental Bio-Systems, Inc. has focused a core of remedial planning and design services directed to support environmental consulting companies. The services relate to remedial technology evaluation and remedial options, development of case closure strategies, bench testing and remedial design. The company provides financial resources and funding for selected remedial services and products to keep environmental projects moving forward, accepting reimbursement from insurance or state funding agencies. EBS also provides remedial training and workshops for consultants and regulators.


EBS works exclusively with consulting companies to evaluate various environmental remediation treatment technologies for groundwater and soil/sludges. Technology evaluations are focused on site-specific issues, including geochemistry, current use, future use, exposure pathways and estimated dose and time factors needed for degradation of the contaminants. EBS works with chemical oxidation technologies (ozone, perozone, Fenton’s Reagent, permanganate, persulfate and other chemical oxidizers which are used primarily to treat chlorinated solvents, petroleum hydrocarbons and other generally volatile and semi-volatile organic compounds such as pesticides/herbicides, PCBs, and PAHs.





Cleanup Design Options and Treatability Testing for In Situ Remediation of Soil and Ground Water


Infusion of dissolved gases in an aquifer can significantly accelerate natural biodegradation for petroleum hydrocarbons, chlorinated solvents and other chemicals. Although bioremediation is generally a slow process, taking a few months to start to see results, it can be an effective and low cost remediation strategy. Enhanced bioremediation can be used to treat some source areas, and off-site migration even in low permeability materials. This technology can also enhance existing pump and treat systems, reducing remediation time significantly. EBS recommends gas infusion technologies, a delivery method for electron acceptors, such as oxygen, for aerobic systems. Different gases such as propane, butane, ethene, methane or others can be used for anaerobic systems. The most successful gas transfer device available is the iSOC. EBS is an iSOC representative for northern California. EBS performs biological and chemical laboratory bench tests as well as microbial profiling. These pre-field activities are highly recommended before starting any in-situ enhanced bioremediation program. A good hydrogeology primer is the USGS Basic Hydrogeology (Water-Supply Paper 2220 by Ralph Heath). For in-situ remediation of chlorinated solvents, the U.S. EPA document provides background information.

EBS and iSOC Products and Services

iSOC™ Brochure (PDF)

EBS iSOC™ Support Products & Services Catalog (PDF-18 page catalog)
Catalog includes regulators, tubing, and comments on operation, iSOC well supplies, security cabinets, and pressure alarms, DO/ORP meters, bioremediation feasibility studies and microcosm bench tests

       bulletiSOC™ Well Supplies (PDF)
       bulletiSOC™ Installation Procedure (PDF)
       bulletiSOC™ Quote Form (PDF)
       bulletiSOC™ Photographs
       bulletiSOC™ Trouble Shooting Matrix (PDF)
       bulletiSOC™ Suggestions and Hints (PDF)
       bulletiSOC™ Contaminant Groups Specific Elements (PDF)
       bulletNatural Attenuation Lab Studies (PDF)
       bulletEPA - Chlorinated Solvents (PDF)
       bulletEPA - Cr(VI) Attenuation Study (PDF)
       bulletBottles Required for Aerobic Lab Analysis (Word document)
       bulletBottles Required for Aerobic Lab Analysis (PDF)
       bulletBottles Required for Anaerobic Lab Analysis (Word document)
       bulletBottles Required for Anaerobic Lab Analysis (PDF)

EBS Bonus:
Free enhanced bioremediation (iSOC) workplans, which can be used to help with the preparation of your site-specific workplan (Word documents).

       bulletAerobic Workplan
       bulletAnaerobic Workplan

Diagrams and drawings for use in your workplans (Adobe Acrobat documents)

       bulletiSOC concrete cut details
       bulletiSOC flow diagram
       bulletiSOC security storage
       bulletiSOC trench detail
       bulletiSOC well box with tanks
       bulletiSOC well details
       bulletiSOC well box details (1 of 2)
       bulletiSOC well box details (2 of 2)
       bulletiSOC Installation diagrams

Material Safety Data Sheets (MSDS) for use in your workplans (Adobe Acrobat documents)

       bulletContaminant Groups and Specific Elements

iSOC Case Studies

Technical Information

       bulletAbstract - 2005-003 Interpretation of Indirect Geochemical Indicators for In-Situ Bioremediation (PDF)
       bulletAbstract - 2005-002 A Case Study of Indirect Geochemical Indicators (PDF)
       bulletAbstract - 2006-003 Passive In-Situ Oxygen and Nutrient Injection Project in Santa Rosa (PDF)
       bulletAbstract - 2006-002 Biosolvent Flushing and High Vacuum Extraction of Heavy Waste Oil (PDF)
       bulletAbstract - 2006-001 Hotspot Well Treatment of Petroleum Hydrocarbons for Site Closure (PDF)
       bulletAbstract - 2005-001 In-Situ Bioremediation in a Hotspot Well in Northern California (PDF)
       bulletAbstract - Enhanced Bioremediation (PDF)
       bulletAbstract - iSOC™ Bioremediation Enhancement Brochure (Courtesy iTi. PDF)
       bulletAbstract - iSOC™ User Guide (Courtesy iTi. PDF)
       bulletAbstract - The iSOC™ Advantage (Courtesy iTi. PDF)
       bulletAbstract - iSOC™ Equipment Setup Quick View (Courtesy iTi. PDF)
       bulletAbstract - iSOC™ Tubing Specification (Courtesy iTi. PDF)
       bulletAbstract - MTBE Removal Using iSOC™ Gas Infusion Technology (PDF)
       bulletAbstract - Co-inFusion of Oxygen & Alkane Gas for Bioremediation of GW Pollutants (Courtesy iTi. PDF)
       bulletAbstract - Gas inFusion™ Introduction and iSOC™ Introduction and Performance (PDF)
       bulletIntrinsic Biodegradation Respiration (Courtesy Sierra Environmental. Click screen to advance slides)
       bulletLetter - Florida Dept. of Environmental Protection - In-situ Submerged Oxygen Curtain (iSOC™) (PDF)

Anaerobic Fermentable Oils: The anaerobic fermentation process (SEE ATTACHED PDF) is a well understood and documented method to remediate many chlorinated solvents (such as PCE and TCE), perchlorate, nitrate, TNT and some heavy metals, such as chromium in soil and groundwater. The EBS Anaerobic Enhancer 60H is a soybean oil-based remediation product that can be used for rapidly creating anaerobic and reducing conditions. As documented on a site in New Mexico, combine fermentable oils with the optional hydrogen infusion using the gPRO (or HiSOC), and contaminant degradation results were up to 50% greater than just the use of fermentable oils alone. 

Laboratory Bench-Scale Services: Commonly overlooked, bench testing provides some of the most important site-specific information about the likelihood of success or failure of an in situ remediation project. EBS performed dozens of bench tests for in situ chemical oxidation (ISCO) (bench tests for Fenton's Reagent, permanganate, persulfate, ozone, and others), enhanced bioremediation (microbiological feasibility studies and microcosm studies) and metals stabilization for numerous consulting companies. These tests are a way to optimize reactant chemistry in the laboratory rather than in the field. In addition, EBS provides bench tests for process treatments for environmental equipment to verify successful results and proper designs.

       bulletBench Testing (PDF)
       bulletNatural Attenuation Lab Studies (PDF)
       bulletAbstract - iSOC Testing Parameters (PDF)
       bulletAbstract - Microbiological Lab Analyses (PDF)
       bulletList of Field Parameters (PDF)

In-Situ Chemical Oxidation (ISCO) Remediation: EBS designs and implements a variety of in situ chemical oxidation (ISCO) processes for soil and groundwater remediation. EBS uses the high-pressure injection of liquid or solid chemical oxidants such as the hydroxyl radical (Fenton's Reagent), hydrogen peroxide, persulfate, potassium, sodium and calcium permanganate, percarbonate, and persulfate to greatly accelerate the complete destruction of petroleum hydrocarbons, chlorinated solvents, MTBE, BTEX, PCBs, PCPs and other organic chemicals. Delivery pressures for liquid oxidants include well and filter gallery trenches (30 to 40 psi), moderate pressure injection rods (200 to 600 psi) to high pressure (3,000 to 5,000 psi) lances. Ozone, a gas, is also used for in-situ remediation in a variety of delivery systems. EBS installs the oxidants with proprietary injection and pumping technologies in specialized sparge points or treatment trenches. Ozone can be injected at low pressure (less than 15 psi) or higher line pressures (40 to 75 psi) for in situ applications. Safety design is an important element of any EBS injection project.

Ozone Treatment Packages: Ozone (O3) is a powerful gas phase oxidizer that can be used to treat volatile organic compounds. Ozone must be generated on-site and the gas cannot be stored; therefore all the ozone gas that is generated must be injected into the subsurface or destroyed using an ozone destruction unit on the ozone generator. The ozone gas can be bubbled into closely spaced injection ports that release the bubbles into the aquifer for remediation. The smaller the bubbles, the more surface area and the faster they can travel through small pore spaces. Pumping the ozone gas through specially designed ozone diffusers can produce micro-bubbles. Advanced oxidation processes refer to when ozone is catalyzed or enhanced by ultra violet light, hydrogen peroxide or other oxidizers, to increase the power of the ozone by producing more hydroxyl radicals. Treatability testing in the laboratory can evaluate the cost benefit of the different ozone enhancements prior to mobilizing into the field.

EBS has designed complete and competitive ozone packages for in-situ and surface treatment of water, including wells, well points, sparge points, and trenches. Installation is available nationwide. Please call for custom services (electronic controllers, other project requirements). With ozone compatible compressors, line pressures from the ozone unit to the sparge points can range from about 40 to 75 psi. Please note: the higher the line pressure, the lower the stability and concentration of the ozone being delivered to an aquifer or vadose zone. Safety design is an important element of any EBS injection project. Please call for details 415-381-5195.

In-Situ Metals Geochemical Fixation or Stabilization: EBS designs and injects chemicals to immobilize toxic soluble metals such as lead, arsenic, hexavalent chromium, cadmium, nickel and others using geochemical fixation. The treated metals become insoluble in the process, reducing the need for expensive excavation and disposal. EBS uses calcium polysulfide, sodium metabisulfite and ferrous sulfate in a proprietary jetting technology. Safety design is an important element of any EBS injection project.

Remediation Equipment: EBS installs and distributes a variety of groundwater and treatment water and soil remedial equipment such as ozone generators, thermal oxidizers, oil water separators, emulsion-splitting plants, evaporators, equipment and chemical storage, sewer treatment discharge plants, car/truck wash systems, industrial wash wastewater recycling systems, advanced oxidation systems, fluidized bed bioreactors, and grease treatment systems.

EBS works with small wineries (<30,000 cases per year) and larger ones who are required to meet water treatment goals for waste water used in the wine making process and equipment washing process. Until relatively recently, this segment of industry has not seen much regulatory oversight, however, water regulators are now focusing on wineries. This water typically has 3,000 to 5,000 mg/L biological oxygen demand (BOD). EBS has developed a modular approach that is flexible for wineries as well as cost effective for discharge to land through irrigation or discharge to a sanitary sewer. Typical waste discharge requirements for discharges of winery waste are available in the link below.

       bulletAbstract - Winery Discharge Requirements (PDF)

Remediation Seminars and Workshops: EBS provides one to two day seminars and workshops on designing in-situ remediation programs. Discussion on in-situ delivery methods, chemical oxidation chemistry, enhanced bioremediation (aerobic and anaerobic), and metals stabilization. The lectures are given by James Jacobs, Chief Hydrogeologist and Fulbright award winner.

Accelerated Surfactant Technology: EBS uses environmentally friendly, naturally occurring surfactants for acceleration of in-situ remediation of free product (both hydrocarbon and chlorinated solvents). By generating micro-droplets, the free product is broken down from one large liquid area into billions of micro-droplets. This transformation increases in surface area, allowing for more rapid degradation by both chemical oxidation and enhanced bioremediation. EBS is a manufacturer's representative for a variety of specialized remediation surfactants.

Air Toxins: Mold, Viruses and Fungi: EBS has assessed and sampled for molds, viruses and fungi at numerous propertiesi. EBS designs and provides ozone/UV systems to destroy molds, viruses and fungi in walls, crawl spaces, ducts and other confined spaces. Depending on residence time or treatment dose, the SARS virus and anthrax can be destroyed using these technologies. In addition, EBS has equipment for industrial, commercial and residential uses that continues to remove air toxins. These state-of-the-art technologies continue to reduce the potential for recurring sick building syndrome by removing odors, air pollutants, mold, viruses and bacteria, once the original air pollutant sources have been removed. These products offer protection of employees and family members.